Expression of simian virus 40-rat preproinsulin recombinants in monkey kidney cells: use of preproinsulin RNA processing signals.

Preproinsulin mRNA in the rat eye.

PURPOSE The goal of this study was to extend the results of previous immunoassay, immunocytochemistry, and in situ hybridization studies showing the presence of insulin-related peptide in the rat retina by confirming the expression of insulin genes in the rat eye. METHODS Total and poly(A)+ RNA were isolated from whole rat eyes, and separately from the retina, choroid, iris, lens, and vitreou...

Normal Mice the Immune Response to Preproinsulin in Expression of Preproinsulin-2 Gene Shapes

Isolation of simian virus 40 recombinants from cells infected with oligomeric forms of simian virus 40 deoxyribonucleic acid.

Oligomeric forms of simian virus 40 (SV40) deoxyribonucleic acid (DNA) were isolated from monkey kidney cells infected with two plaque morphology mutants of SV40. Recombinant, large clear-plaque-type SV40 was produced in cells productively infected with oligomeric forms of SV40 DNA.

Cytological and Cytochemical Studies of Green Monkey Kidney Cells Infected in Vitro with Simian Virus 40

Cytological and cytochemical studies of green monkey kidney cells infected with SV40 virus indicated that the type of lesion produced was influenced by the multiplicity of infection and that the lesions appeared later and progressed more slowly when the inoculum was diluted. The earliest change consisted of enlargement of ribonucleoprotein-containing spherules in the nucleolus (nucleolini). Thi...

Protein synthesis in Simian virus 40-infected monkey cells.

The proteins of purified Simian virus 40 (SV40) were examined by sodium dodecyl sulfate-acrylamide gel electrophoresis and compared with the polypeptides synthesized in SV40-infected monkey cells. Purified virions contain two major components, with molecular weights of 44,000 and 31,000. Together they make up 83% of the total virion proteins. In addition, the virus contains 12 minor polypeptide...